Molecular Typing of Brucella melitensis and B. abortus from Human Blood Samples Using PCR-RFLP Method

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molecular typing of brucella melitensis and b. abortus from human blood samples using pcr-rflp method

conclusions the results of this study showed that pcr–rflp technique was a fast and applicable method especially for separation, detection and differentiation between species of b. melitensis and b. abortus biovars in blood sample. also the presented data showed that b. melitensis biovar 1 was the prevalence biovar. background brucella is an intracellular parasite of the disease brucellosis thr...

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Detection of Brucella melitensis and Brucella abortus strains using a single-stage PCR method

  Brucella melitensis and Brucella abortus are of the most important causes of brucellosis, an infectious disease which is transmitted either directly or indirectly including consuming unpasteurized dairy products. Both strains are considered endemic in Iran. Common diagnostic methods such as bacteriologic cultures are difficult and time consuming regarding the bacteria. The aim of this study w...

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MLVA16 Typing of Portuguese Human and Animal Brucella melitensis and Brucella abortus Isolates

To investigate the epidemiological relationship of isolates from different Portuguese geographical regions and to assess the diversity among isolates, the MLVA16(Orsay) assay (panels 1, 2A and 2B) was performed with a collection of 126 Brucella melitensis (46 human and 80 animal isolates) and 157 B. abortus field isolates, seven vaccine strains and the representative reference strains of each s...

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detection of brucella melitensis and brucella abortus strains using a single-stage pcr method

brucella melitensis and brucella abortus are of the most important causes of brucellosis, an infectious disease which is transmitted either directly or indirectly including consuming unpasteurized dairy products. both strains are considered endemic in iran. common diagnostic methods such as bacteriologic cultures are difficult and time consuming regarding the bacteria. the aim of this study was...

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Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

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ژورنال

عنوان ژورنال: Jundishapur Journal of Microbiology

سال: 2013

ISSN: 2008-3645,2008-4161

DOI: 10.5812/jjm.7197